CD177 antibody (FITC)

Catalog No. ABIN119766

This anti-CD177 antibody is a Mouse Monoclonal antibody detecting CD177 in FACS. Suitable for Human.

Quick Overview for CD177 antibody (FITC) (ABIN119766)

Target: CD177 (CD177 Molecule (CD177))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD177 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MEM-166

Product Details anti-CD177 Antibody (FITC)

Specificity: This antibody recognises human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97 % of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). Antibody reactivity and working conditions may vary between species.

Purification: Protein G affinity chromatography

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (use 10 μL of neat - 1/10 diluted antibody to label 10^6 cells in 100 μL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS with 0.09 % sodium azide as preservative and 1 % BSA as stabelizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background: Cd177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97 % of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN).Synonyms: HNA-2a, HNA2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, PRV1, Polycythemia rubra vera protein 1

Gene ID: 57126

NCBI Accession: NP_065139

UniProt: Q8N6Q3